Skip to main content
Entomology 2020 Main banner
Icon Legend
This session is not in your schedule.
This session is in your schedule. Click again to remove it.
Presentation Icons
Plenary
Plenary
Livestream
Livestream
On-Demand
On-Demand
Student Competition
Student Competition
Video Chat
Video Chat
Panel Discussion
Panel Discussion
Mixer
Mixer
Poster Icons
Poster
Poster
Student Competition
Student Competition
ViewAttendees 6

MUVE

Student Competition 10-Minute Paper

Undergrad 10-min: MUVE

Discovering and identifying single nucleotide polymorphisms (SNPs) in stable flies (Stomoxys calcitrans L.)

On-Demand
Student Competition On-Demand
Stable flies, Stomoxys calcitrans L., are a blood-feeding livestock pest that harm livestock and tourist industries annually. Stable flies develop in a variety of organic material and are known to disperse over large distances leading to questions about their origin each season. Despite their economic impact, there is still little known about their genetic variation except for evidence of minimal variation in mitochondrial and nuclear sequences. Single nucleotide polymorphisms (SNPs) are markers that can be used for a variety of studies from genome-wide association studies to pathogen prevalence or identification studies. Here, we test the hypothesis that a significant amount of genetic variation exists within the genomic DNA of stable flies. Our objective was to identify high-fidelity and diagnostic genetic markers that can be used for further research. To discover SNPs, 192 specimens were field-collected globally. After genomic DNA was extracted, a quantitative reduced representation sequencing (OmeSeq/qRRS) of the genome was performed. Resulting sequence reads were quality-filtered using ngsComposer, while SNP calling and filtering was performed using GBSapp by aligning reads to the stable fly reference genome. Multiple stringent filtering parameters and quality controls were used to generate 9,343 high-fidelity and diagnostic biallelic SNPs that map to a single locus. These SNPs will be discussed and provide the opportunity for continued genomic research.