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PBT

Student Competition 10-Minute Paper

Undergrad 10-min: PBT, General

HIF signaling and lactate levels during ontogeny of the third larval instar of Drosophila melanogaster

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During development, larval Drosophila melanogaster may exhibit expression of Hypoxia-Inducible factor (HIF) and lactate accumulation. This might indicate periods of tissue hypoxia, or alternatively could indicate developmental roles of HIF signaling and aerobic glycolysis. We used a strain of D. melanogaster that expresses a green fluorescent protein-tagged oxygen-dependent domain of HIF to assess HIF signaling at four time points during the third instar in the central nervous system and muscle. At similar time points, we measured lactate levels. Both HIF signaling and lactate levels were highest at the beginning of the third instar, declining over time within the instar. This pattern could indicate that HIF-signaling and aerobic glycolysis supports rapid growth of tissues including tracheae after molting; alternatively, elevated HIF signaling and lactate early in the instar might indicate that molting is associated with a disruption of oxygen transport. The research was partially supported by NSF IOS 1256745.