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P-IE

Section Symposium

Category: Section Symposium

Fundamental Biology to Practical Management of Ambrosia Beetles in Horticultural Tree Agroecosystem Poster Session

1: Transcriptome profiling of the antennae from the ambrosia beetle Xylosandrus germanus (Coleoptera: Curculionidae)

Poster

    Presenting Author(s)

  • Nisha Patwa

    Post-Doctoral Research Associate
    USDA-ARS
    Wooster, Ohio, United States

    • Co-Author(s)

  • Chris M. Ranger

    Research Entomologist
    USDA-ARS
    Wooster, Ohio, United States

  • MR

    Mike E. Reding

    Research Entomologist
    USDA
    Wooster, Ohio, United States

The Ambrosia beetle, X. germanus is native to southeast Asia, but now established in >30 US states. It attcaks horticultural trees and is associated with branch dieback and tree death. Ethanol is a key attractant for X. germanus, and trees emitting ethanol in response to a variety of abiotic and biotic stressors are preferentially attacked. Our objective is to profile the transcriptome of antennae to assess olfactory genes associated with the detection of ethanol. Here we report on techniques being established for exposing specimens to ethanol vapors, dissecting antennae, isolating RNA, and optimizing RT-qPCR methods. A cDNA library is being established for sequencing, bioinformatic analysis of the sequences and validation of potent transcripts expression.