RNA interference (RNAi) is a post-transcriptional gene silencing mechanism that is initiated by the presence of double-stranded RNA (dsRNA), resulting in degradation of a target mRNA and failure of the corresponding protein production. Although RNAi technology is a promising tool for insect pest management, the efficacy of RNAi varies among different insect orders and also depends on various factors. G-protein coupled receptors (GPCRs) are involved in almost all physiological processes including neurotransmitters, and hormones, etc. Insect GPCRs as a target for new specific class of insecticides holds great promise. We recently used a novel GPCR-based screening method using a GPCR of insect peptides, and successfully screened small peptides to be evaluated as bioactive agents to control the pest. The mechanism of the technology selects peptides that strongly bind to the receptor, thus blocking the specific physiological functions. We refer to this mode of action as Receptor interference (Receptor-i). We explore the feasibility and implementation of these new tools for application to control SWD.
