Evaluation of fast pyrolisis bio-oils as a potential source of novel biopesticides

The goal of our ongoing project is the development of a sustainable source of bioadvantaged pesticides from byproducts from waste plant materials processed with Catalytic Fast Pyrolysis (CFP) for the production of biofuels. As a first step, we used larval oriental fruit moth Grapholita molesta (Busck) (OFM) and adult spotted-wing drosophila, Drosophila suzukii (Matsumura) (SWD) as model organisms. We screened 10 fractions of CFP developed from wood pulp at 80 mg/ml and 20 mg/ml with the balance of material made up of 70% acetone. Five and ten replicates were performed for each dose/application for spotted-wing drosophila  and oriental fruit moth, respectively. OFM 1st-2nd instar were evaluated in a poison diet bioassay while SWD adult mortality was evaluated in response to exposure to residuals. All 10 fractions caused 100% mortality of OFM at concentrations of 80 mg/ml. Five of the 10 fractions caused 100% mortality for SWD at 80mg/ml. Mortality for the 20mg/ml doses ranged from 10-95% and