Circadian rhythm analysis of Drosophila melanogaster in an integrated experimental unit

Presenting Author(s)

Ivan Filippov

London, ENG

Co-Author(s)

Bill Budenberg

Founder
Zantiks Ltd
Cambridge, United Kingdom
AJ

Alexandra Jarrett, n/a

Intern
Zantiks Ltd
London, England, United Kingdom

Circadian rhythm in insects have often been previously studied by monitoring infrared beam crossings, such as the DAM systems (Trikenetics). We present an automated, temperature-controlled, light-tight behavioral testing unit (the Zantiks MWP) equipped with video-tracking as an alternative system for circadian studies.

Using this unit, we demonstrate circadian rhythm entrainment and phase shift in Drosophila melanogaster through utilising lighting and temperature stimuli. A live tracking system monitors distance travelled and pixel change between frames, allowing recording and identification of micro-movements, which is not possible with beam-crossing systems.

Multiple light-dark cycles were tested in a single unit by introducing light-tight barriers between arenas in the testing chamber, as well as targeted light pulses for phase change. The data was then analysed in R using the Rethomics package. We demonstrate the capacity of the unit to perform automated, easily replicated circadian experiments.